Lipids

Lipids: An Overview

  • Lipids are a group of biological molecules that are insoluble in water but are soluble in organic solvents.
  • The main biological roles of lipids include energy storage, serving as structural components of cell membranes, and participating in signalling processes.
  • Lipids can be subdivided into different categories, such as triglycerides, phospholipids and steroids.

Triglycerides

  • Triglycerides are formed from one glycerol molecule and three fatty acids.
  • Ester bonds link the fatty acids and the glycerol molecule together. This process is a condensation reaction resulting in the formation of water.
  • They are the main form of energy storage in animals due their high energy content.
  • Fatty acids can be saturated (no double bonds between carbon atoms) or unsaturated (one or more double bonds present).

Phospholipids

  • Phospholipids are similar to triglycerides but one of the fatty acids is replaced by a phosphate group.
  • As a result, phospholipids have a hydrophilic ‘head’ and two hydrophobic ‘tails’.
  • The dual nature of these molecules forms the basis for the creation of cell membranes, where they arrange into a bilayer with the tails facing inwards.

Steroids

  • Steroids are lipids characterised by a carbon skeleton consisting of four fused rings.
  • They have diverse functions in the body. For example, cholesterol is a type of steroid that is a key component of cell membranes and is also used to synthesise other steroids.

Tests for Lipids

  • The emulsion test is a common test for lipids. Mix the sample with ethanol, then add water. If a milky-white emulsion forms on the top, lipids are present.
  • Lipids can also be detected using the sudan IV test, where lipid-containing samples turn red in the presence of the Sudan IV dye.