Photosynthesis
Photosynthesis
Light-Dependent Reaction:
- Chlorophyll absorbs light, leading to photoionisation of chlorophyll.
- Energy from electrons released during photoionisation is conserved in the production of ATP and reduced NADP.
- ATP production involves electron transfer associated with the electron transfer chain, proton passage across chloroplast membranes, and catalysis by ATP synthase (chemiosomotic theory).
- Photolysis of water produces protons, electrons and oxygen.
- Light-dependent reaction provides reduced NADP and ATP for light-independent reaction.
Light-Independent Reaction:
- Carbon dioxide reacts with ribulose bisphosphate (RuBP) to form two molecules of glycerate 3-phosphate (GP), facilitated by enzyme rubisco.
- ATP and reduced NADP from light-dependent reaction reduce GP to triose phosphate.
- Some of the triose phosphate regenerates RuBP in the Calvin cycle.
- Some triose phosphate is converted to useful organic substances.
Other Important Information:
- Should be able to identify environmental factors that limit photosynthesis rate.
- Should be able to evaluate data on agricultural practises used to mitigate limiting factors’ effects.
- Photosynthesis rate investigation methods and techniques should be revised.
- Ability to interpret graphs representing multiple limiting factors is important.
Practical Skills:
- Chromatography use to examine plant pigments.
- Investigating the impacts of factors on dehydrogenase enzymes.