Recombinant DNA Technology

  • Recombinant DNA technology examples include genetic engineering of crops, production of recombinant proteins, and gene therapy.
  • The method involves the use of cDNA, reverse transcriptase, restriction enzymes, gene machines, and DNA amplification.
  • cDNA is synthesised from mRNA using reverse transcriptase.
  • Restriction enzymes are used to cut the DNA at specific sequences.
  • Gene machines are used to artificially synthesise DNA sequences.
  • DNA amplification techniques like PCR are used to generate multiple copies of a specific DNA fragment.
  • A flow chart diagram can be used to illustrate the various stages of the process.