Recombinant DNA Technology

• Recombinant DNA technology examples include genetic engineering of crops, production of recombinant proteins, and gene therapy.
• The method involves the use of cDNA, reverse transcriptase, restriction enzymes, gene machines, and DNA amplification.
• cDNA is synthesised from mRNA using reverse transcriptase.
• Restriction enzymes are used to cut the DNA at specific sequences.
• Gene machines are used to artificially synthesise DNA sequences.
• DNA amplification techniques like PCR are used to generate multiple copies of a specific DNA fragment.
• A flow chart diagram can be used to illustrate the various stages of the process.