Recombinant DNA Technology
- Recombinant DNA technology examples include genetic engineering of crops, production of recombinant proteins, and gene therapy.
- The method involves the use of cDNA, reverse transcriptase, restriction enzymes, gene machines, and DNA amplification.
- cDNA is synthesised from mRNA using reverse transcriptase.
- Restriction enzymes are used to cut the DNA at specific sequences.
- Gene machines are used to artificially synthesise DNA sequences.
- DNA amplification techniques like PCR are used to generate multiple copies of a specific DNA fragment.
- A flow chart diagram can be used to illustrate the various stages of the process.