Calibration Plot

Section 1: Understanding Calibration Plots

  • A calibration plot is created to establish the relationship between the response of an instrument and the concentration of the analyte.
  • This technique is critical in instrumental analysis.

Section 2: Components of a Calibration Plot

  • Analyte concentration: On the x-axis, one measures the different concentrations of the known solutions.
  • Instrument response: The y-axis reflects the signal or response the instrument gives depending on the concentration.

Section 3: Creating Calibration Plots

  • Known concentrations of the analyte, or standards, are used to create a calibration plot.
  • Each standard is analysed using the instrument to measure the instrument response.
  • The response is plotted against the concentration to establish a calibration curve.

Section 4: Interpreting Calibration Plots

  • The calibration curve can be used to determine the concentration of unknown samples.
  • The response of the sample is measured and compared to the calibration curve.
  • The calibration curve shows an expected signal for each concentration, which can be extrapolated or interpolated for unidentified sample sizes.

Section 5: Practical Application of Calibration Plots

  • Calibration plots are crucial in analytical laboratories to identify the concentration of substances within a sample.
  • The plots can help in fields such as environmental monitoring, pharmaceutical testing and forensic analysis.

Section 6: Uncertainty in Calibration Plots

  • All measurements have some degree of uncertainty, which must be considered in calibrations.
  • Measurement errors can be minimised through careful calibration and repeated measurements.

Section 7: Safety and Ethical Considerations with Calibration Plots

  • When creating calibration plots, always adhere to the relevant safety protocols, especially when handling toxic or harmful analytes.
  • Calibration plots must always be carried out with precision and integrity to ensure accurate and reliable results.